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Heinz Hopf who plays one of the main crooks is as good as always but the rest of the cast is nothing to cheer about. I didnt like this movie at all.

No suspense and no real action. Only reason to watch this movie is because it is Swedish and very hard to come by.

I will never watch it again. Sign In. Keep track of everything you watch; tell your friends. Full Cast and Crew. Release Dates. Official Sites. Company Credits.

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Writer: Olov Svedelid. Added to Watchlist. Comic-Con Home Top Moments. Major Comic-Con Home News. Swedish films. The Best Swedish Films. Share this Rating Title: Smutsiga fingrar 4.

Use the HTML below. You must be a registered user to use the IMDb rating plugin. Photos Add Image. Should penetration occur only so far?

Asking these types of questions can be helpful as there are some aspects about anal stimulation and penetration that for some folks are considered taboo and are a challenge to talk about.

Anal stimulation can be a fun and pleasurable way to explore your sexuality as long as you take a few precautions. While your anal sphincters the muscles that keep your bum puckered up can stretch to accommodate various sizes and shapes, they understandably have their limits.

But no pun intended as a general rule, folks can usually and safely insert objects up to about six and a half inches long. A bit of anal anatomy might help you gain a clearer picture of the possibilities of anal stimulation, making these adventures less painful and more enjoyable.

The anus and rectum the area just inside the anus are together about six and a half inches long. It also has two sphincters that are typically contracted very tightly to keep waste from making unexpected appearances!

The sphincter muscles can squeeze involuntarily, which can pull objects into the anus. This could result in a trip to a health care provider to have an object removed — a decidedly unsexy and awkward ending to your exploration!

Using a thick water- or silicone-based lube can also help get things moving — literally! The tissue of the anus is thin and taut, making transmission more likely than vaginal activities.

Debby Herbenick. You could also have a chat with a health care provider or a health promotion professional to get some more of your questions answered.

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All About Alice! Go Ask Alice! Get Alice! In Your Box. Ann Sermee : just kidding; I'm a guy 2 Dear Alice, I recently posted the question about the finger in the anus.

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The gag-knuckle zinc finger in the HIV NC protein is the target of a class of drugs known as zinc finger inhibitors. These fingers are present in a diverse group of proteins that frequently do not share sequence or functional similarity with each other.

The best-characterized proteins containing treble-clef zinc fingers are the nuclear hormone receptors. The zinc ribbon fold is characterised by two beta-hairpins forming two structurally similar zinc-binding sub-sites.

The canonical members of this class contain a binuclear zinc cluster in which two zinc ions are bound by six cysteine residues. These zinc fingers can be found in several transcription factors including the yeast Gal4 protein.

It is used in mammals for antiviral defense. Various protein engineering techniques can be used to alter the DNA-binding specificity of zinc fingers [18] and tandem repeats of such engineered zinc fingers can be used to target desired genomic DNA sequences.

Typical engineered zinc finger arrays have between 3 and 6 individual zinc finger motifs and bind target sites ranging from 9 basepairs to 18 basepairs in length.

Arrays with 6 zinc finger motifs are particularly attractive because they bind a target site that is long enough to have a good chance of being unique in a mammalian genome.

Engineered zinc finger arrays are often fused to a DNA cleavage domain usually the cleavage domain of FokI to generate zinc finger nucleases.

Such zinc finger-FokI fusions have become useful reagents for manipulating genomes of many higher organisms including Drosophila melanogaster , Caenorhabditis elegans , tobacco , corn , [23] zebrafish , [24] various types of mammalian cells, [25] and rats.

If a homologous DNA "donor sequence" is also used then the genomic locus can be converted to a defined sequence via the homology directed repair pathway.

The majority of engineered zinc finger arrays are based on the zinc finger domain of the murine transcription factor Zif, although some groups have used zinc finger arrays based on the human transcription factor SP1.

Zif has three individual zinc finger motifs that collectively bind a 9 bp sequence with high affinity.

In and , a number of groups used phage display to alter the specificity of a single zinc finger of Zif The most straightforward method to generate new zinc finger arrays is to combine smaller zinc finger "modules" of known specificity.

The structure of the zinc finger protein Zif bound to DNA described by Pavletich and Pabo in their publication has been key to much of this work and describes the concept of obtaining fingers for each of the 64 possible base pair triplets and then mixing and matching these fingers to design proteins with any desired sequence specificity.

Another method uses 2-finger modules to generate zinc finger arrays with up to six individual zinc fingers. A recent study demonstrated that a high proportion of 3-finger zinc finger arrays generated by modular assembly fail to bind their intended target with sufficient affinity in a bacterial two-hybrid assay and fail to function as zinc finger nucleases , but the success rate was somewhat higher when sites of the form GNNGNNGNN were targeted.

A subsequent study used modular assembly to generate zinc finger nucleases with both 3-finger arrays and 4-finger arrays and observed a much higher success rate with 4-finger arrays.

Numerous selection methods have been used to generate zinc finger arrays capable of targeting desired sequences.

Initial selection efforts utilized phage display to select proteins that bound a given DNA target from a large pool of partially randomized zinc finger arrays.

This technique is difficult to use on more than a single zinc finger at a time, so a multi-step process that generated a completely optimized 3-finger array by adding and optimizing a single zinc finger at a time was developed.

A promising new method to select novel 3-finger zinc finger arrays utilizes a bacterial two-hybrid system and has been dubbed "OPEN" by its creators.

This system was developed by the Zinc Finger Consortium as an alternative to commercial sources of engineered zinc finger arrays.

It is somewhat difficult to directly compare the binding properties of proteins generated with this method to proteins generated by modular assembly as the specificity profiles of proteins generated by the OPEN method have never been reported.

Proteins containing these domains include:. From Wikipedia, the free encyclopedia. Small structural protein motif found mostly in transcriptional proteins.

Biology portal Technology portal. The Journal of Biological Chemistry. Elements for interactions between macromolecules".

Annual Review of Biochemistry. Bibcode : PNAS.. Journal of Molecular Biology. Bibcode : Sci Bibcode : Natur. Molecular and Cellular Biology.

Current Opinion in Structural Biology. Trends in Biochemical Sciences. Nucleic Acids Research. On: SciTechDaily, April 14th, Nature Reviews.

Drug Discovery. Bibcode : PNAS Soon, they're chased all over Stockholm by the cruel but suave ring leader Harry and his thugs. Among the wildness is a fight in a brothel filled with naked girls, and when Servus, one of the thugs, fails to kill our heroes, Harry has his men kill Servus with a razor blade equipped glove.

Oh, and the heroine is raped by a guy sucking on a comforter. Written by Sujit R. This movie is pretty lame.

Interesting story but boring. Heinz Hopf who plays one of the main crooks is as good as always but the rest of the cast is nothing to cheer about.

I didnt like this movie at all. No suspense and no real action. Only reason to watch this movie is because it is Swedish and very hard to come by.

I will never watch it again. Sign In. Keep track of everything you watch; tell your friends. Full Cast and Crew. Release Dates. Official Sites.

Company Credits. Technical Specs. Plot Summary. Plot Keywords. Parents Guide. External Sites. User Reviews.

User Ratings. External Reviews. Metacritic Reviews. Photo Gallery. Trailers and Videos. Crazy Credits. Alternate Versions.

Rate This. Soon, they're chased all over Stockholm by the cruel but suave Director: Arne Mattsson. Writer: Olov Svedelid.

Added to Watchlist. Comic-Con Home Top Moments. Major Comic-Con Home News.

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